Identifying neurons in research relies on antibodies and surface markers that target specific proteins expressed in neuronal cells. These tools enable precise labeling, sorting, and functional analysis of neurons in both fixed and live samples.
đ§ Marker Categories:
- Intracellular Markers: Target proteins within the nucleus or cytoplasm (e.g., NeuN, MAP2, ÎČIII-Tubulin)
- Used for fixed tissue analysis and neuron quantification
- Surface Markers: Bind to membrane proteins (e.g., NCAM, L1CAM, Thy-1)
- Enable live cell sorting and real-time monitoring
đŹ Applications:
- Immunohistochemistry & Immunofluorescence: Visualize neuron morphology and distribution
- Flow Cytometry: Sort neuronal subpopulations with high specificity
- Disease Modeling: Detect neuronal damage and synaptic changes in neurodegenerative conditions
- Stem Cell Differentiation: Use marker combinations (e.g., CD49fâș/CD200âș) to isolate neurons from iPSC-derived cultures
đ Advantages:
- High specificity and expression levels
- Compatibility with live and fixed samples
- Functional diversity for various research needs
These tools are foundational for neuroscience, enabling researchers to explore neural circuits, cell fate, and disease mechanisms with precision. Want help designing a marker panel or comparing labeling techniques? Iâd be happy to assist.
